12 December 2017 1 4K Report

Hello all,

I've been trying for a while now to crosslink dopamine to chitosan using various means, more recently I have been using glutaraldehyde.

My electrode is made in house using an ink from Gwent. It seems that once printed the ink is very susceptible to attack from a variety of solvents, such as acetone, toluene, etc. I've mainly used alcohols (solvent cast) and electropolymerisation/deposit to layer my electrodes.

I've been trying to link the two amine groups of the chitosan and dopamine but have been having issues. This most recent one affects the structural stability of the working area of the electrode.

Protocol:

  • clean in 3:1 Piranha for 15 secs, rinse x3 and dry under nitrogen.
  • Solvent cast a co-polymer of graphene oxide and chitosan, dry in oven at 30 degrees centigrade for 1 hour.
  • wash off excess and dry under nitrogen
  • place working area of electrode in 5% Glutaraldehyde at 37 degrees centigrade for 2 hours.
  • Wash off excess and dry in oven at 30 degrees centigrade for 1 hour.
  • Now the next step would be to incubate in a poly dopamine solution pH 9.0 at 37 degrees centigrade for 3 hours but the electrodes seem to 'crack', and lift from the substrate surface in some cases, almost slightly desiccated?

    Can anyone point out the error in my protocol or offer some useful advice?

    I have previously tried electrodepositing chitosan to the electrode, which resulted in uneven coating due to bubbling at the surface.

    Regards,

    Craig

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