Craig Mcbeth

12 Questions 9 Answers 0 Followers

Questions related from Craig Mcbeth

Use of HEPES in bicarbonate free media, what are the potential issues?

Hello all, I have used HEPES in the past to maintain my cells outside of CO2 incubators for extended periods of time, however some of my recent work would best be performed if the bicarbonate...

11 November 2019 1,895 3 View

Reference electrodes - is electrolyte required?

Hey all, I have used a standard Ag/AgCl electrode for a while now but I've just moved institutions and a lab member is stating the electrolyte is not required for aqueous voltammetric analysis,...

01 January 2019 6,587 5 View

SPCE - improving mediated carbon electrode sensitivity?

Hello all, I'm looking to improve the sensitivity of a mediated carbon electrode. The mediating compound is being added via solvent casting post electrode printing, so any additional treatment/...

04 April 2018 1,139 4 View

Why doesn't my cobalt phthalocyanine electrode look similar to that of previous literature?

Hello all, I have been trying to modify a plain printed carbon electrode with cobalt(II) phthalocyanine and I have found my CV does not look like any of the CVs in literature. I have attached a...

02 February 2018 1,900 3 View

Crosslinking polymer and mediator, printed electrode keeps 'cracking' and separating from substrate using glutaraldehyde, solutions?

Hello all, I've been trying for a while now to crosslink dopamine to chitosan using various means, more recently I have been using glutaraldehyde. My electrode is made in house using an ink from...

12 December 2017 4,447 1 View

How to carboxylate carbon nanotubes?

Hello all, I've been trying to functionalise some CNTs with a carboxylic acid side-chain but I've been having difficulty with my end-product, resulting in negligible yield. My current protocol is...

03 March 2017 4,072 6 View

Is it possible to perform double solvent casting on a printed electrode?

Hello all, For the past week I have been working on a new electrode, the process however uses a double solvent cast in the construction of the electrode. Initially a layer of CNT + Nafion in...

02 February 2017 6,374 2 View

Electrochemical quantification of hydrogen peroxide in an aqueous solution containing cobalt chloride, is this possible?

I have been trying to measure extracellular hydrogen peroxide in basic MEM using electrochemical means. I have been inducing a mimetic state of hypoxia using cobalt chloride hexahydrate and have...

08 August 2016 975 2 View

Looking for studies focusing on the assessment of culture media using electrochemical methods?

Hello all, I'm trying to find some literature on the electrochemical quantification of certain metabolites, amino acids or anything really, in cell culture media e.g. DMEM, EMEM, RPMI etc. Ideally...

04 April 2016 3,348 4 View

Irregular cyclic voltammogram, low/no peaks, what am I doing wrong?

Hello everyone, I'm rather new to electrochemistry on the whole so I'm struggling to trouble shoot this issue. I have been experiencing potential issues with the data I have received from my CV...

04 April 2016 8,078 5 View

Griess reagent issues/ queries; no observable colouration, why?

Hello all, I've been trying to perform a Nitrite/ Griess assay on isolated cell media but no detectable traces of Nitrite is present. The media itself is phenol red-free MEM supplemented with...

05 May 2015 1,611 5 View

Biomarker studies for cell culture supernatant.

I've been searching for any studies relating to the study of cell culture supernatant and the potential biomarkers that may present within unprocessed media. I keep running into paywalls, dead...

10 October 2014 9,528 6 View