How to calculate the gas composition for a heat treatment where a very low oxygen partial pressure is needed?

I'm interested in a selective oxidation in an austenitic stainess steel so that Chromia rather then ferrous oxide is formed on top of my metal. I know I should have a value of pO2 around...

16 December 2020 8,541 7 View

Why do protein and mRNA levels not always correlate with each other?

Hello, I have performed WB analysis and qPCR analysis to analyze the protein level and the rispective gene relative expression of mTOR, GBA1, LC3 genes in brain of mutant mice. I observed a...

01 December 2020 4,486 2 View

Factor analysis with idependent qualitative variables and dependent quantitative variables ?

Hello, I have a set of independent qualitative variables related to households (type of house, type of windows, presence of pets, ...), and dependent quantitative variables (number of arthropods...

19 April 2020 1,788 11 View

Why does LC3-II not increase after CQ treatment in vivo?

I have administered CQ treatment (50 mg/kg) in twelve-months-old C57BL/6J mice to assess autophagic flux. I validated this protocol on 3-months-old mice first, obtaining an accumulation of LC3II...

05 April 2020 5,018 3 View

What could be the advantages of annealing BaTiO3 in vacuum?

I am evaluating if annealing barium titanate in vacuum could have any benefits.

27 January 2020 4,329 1 View

Cas9 containing plasmid toxic for bacteria?

Hello everyone, I am struggling to obtain an overnight/culture for E.coli Stbl3 bacteria transformed with a plasmid containing the Cas9 enzyme and a GFP donor. Although the bacteria grow well in...

19 January 2020 2,245 3 View

Why lentiviral transduction doesn't work?

Hi everybody. I have a problem trying to overexpress by plasmid the gene of my interest (ffar4 for GPR120). I'm able to infect T293 (they appear totally green), then i filtered and centrifuged the...

28 July 2019 5,831 3 View

Experience with service for gene expression with microarray ?

I am looking for a company which can provide a service for several microarrays for gene expression and subsequent results analyses. Does anyone have any experience to share? E.g. Recommending or...

25 March 2019 1,015 3 View

Heat killing of Bordetella species?

13 March 2018 6,065 2 View

How simulate the behavior of a reinforced concrete beam with a B21 element type?

Hi everyone, I am modelling a reinforced beam under impact load. I chose to model it with element type B21. Any suggestion about how to define material's property in order to simulate...

31 January 2018 7,908 4 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View