please help me! I'm staining splenocytes with mitotracker deep red. Is it possible that I get some unstained cells also in the stained sample?
I'm currently working with mouse uterine organoids and I've seen that many authors perform organoid passaging using mechanical or enzymatic methods to dissolve the Matrigel while breaking the...
01 July 2024 381 2 View
I have an antibody specific just for ICC and WB but I should use the same antibody to perform an IHC staining. Is it possible to use this ab also in IHC?
15 May 2024 6,392 3 View
Hi everyone, I'm a PhD student at CAST, Analytical Biochemistry and Proteomics Research Unit, University, "G. d'Annunzio" of Chieti-Pescara. We currently use the Thermo Fisher Orbitrap Fusion...
10 April 2024 9,346 0 View
Title: Impact of Patents on Innovation in Language Technology Description: This research examines the influence of patents on innovation in language technology. It explores the balance between...
09 April 2024 9,061 1 View
How can we navigate the ethical challenges of employing AI in surveillance systems for cybercrime prevention, particularly concerning privacy rights? What design principles and safeguards should...
22 March 2024 5,267 3 View
Hi, I have to extract genomic DNA from plasma samples and will use the Qiamp DNA mini and blood kit (QIAGEN). At the moment the plasma samples are stored at -80° and they were obtained from...
04 March 2024 9,438 1 View
I have started to work with BV2 cells. First, I would like to condition the cells with DMEM 1X. The day after passing the cells, most had adhered to the flask surface; after another two days, most...
29 January 2024 4,450 0 View
Hi, I need to prepare stock solutions for Collagenase I from Sigma (C0130). The provider suggests to prepare solutions in TESCA buffer (50 mM TES, 0.36 mM CaCl2, pH 7.4 at 37 degrees). I tried to...
09 November 2023 2,239 2 View
Hello everyone, I'm dealing with some issues regarding resuspension and use of a Roche DNAse I that we purchased from Merck (Ref:10104159001). The product is purchased as a lyophilized vial (100...
08 November 2023 4,859 0 View
Hi everyone, I transduced my HEK cells with a viral vector containing mRuby as reporter, and now I would like to detect mRuby protein via Western Blot on my cells lysate. Apparently there is no...
05 October 2023 7,336 0 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...
19 July 2024 3,906 0 View