How to calculate the gas composition for a heat treatment where a very low oxygen partial pressure is needed?

I'm interested in a selective oxidation in an austenitic stainess steel so that Chromia rather then ferrous oxide is formed on top of my metal. I know I should have a value of pO2 around...

16 December 2020 8,541 7 View

Why do protein and mRNA levels not always correlate with each other?

Hello, I have performed WB analysis and qPCR analysis to analyze the protein level and the rispective gene relative expression of mTOR, GBA1, LC3 genes in brain of mutant mice. I observed a...

01 December 2020 4,486 2 View

Factor analysis with idependent qualitative variables and dependent quantitative variables ?

Hello, I have a set of independent qualitative variables related to households (type of house, type of windows, presence of pets, ...), and dependent quantitative variables (number of arthropods...

19 April 2020 1,788 11 View

Why does LC3-II not increase after CQ treatment in vivo?

I have administered CQ treatment (50 mg/kg) in twelve-months-old C57BL/6J mice to assess autophagic flux. I validated this protocol on 3-months-old mice first, obtaining an accumulation of LC3II...

05 April 2020 5,018 3 View

What could be the advantages of annealing BaTiO3 in vacuum?

I am evaluating if annealing barium titanate in vacuum could have any benefits.

27 January 2020 4,329 1 View

Cas9 containing plasmid toxic for bacteria?

Hello everyone, I am struggling to obtain an overnight/culture for E.coli Stbl3 bacteria transformed with a plasmid containing the Cas9 enzyme and a GFP donor. Although the bacteria grow well in...

19 January 2020 2,245 3 View

Why lentiviral transduction doesn't work?

Hi everybody. I have a problem trying to overexpress by plasmid the gene of my interest (ffar4 for GPR120). I'm able to infect T293 (they appear totally green), then i filtered and centrifuged the...

28 July 2019 5,831 3 View

Experience with service for gene expression with microarray ?

I am looking for a company which can provide a service for several microarrays for gene expression and subsequent results analyses. Does anyone have any experience to share? E.g. Recommending or...

25 March 2019 1,015 3 View

Heat killing of Bordetella species?

13 March 2018 6,065 2 View

How simulate the behavior of a reinforced concrete beam with a B21 element type?

Hi everyone, I am modelling a reinforced beam under impact load. I chose to model it with element type B21. Any suggestion about how to define material's property in order to simulate...

31 January 2018 7,908 4 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View