I'm analyzing flow data with FloJo.
The little square left of the sample id, whose color indicates the compensation matrix utilized, turned filled, instead of only gridded and I cannot analyze it anymore.
Does anyone know what does that indicates and how to correct it?
Hope I understood you correctly.
Load the same sample in FlowJo 5.7 (works in XP mode) if you are using any newer FlowJo version. The same can happen in 5.7 but you can just click T (transform) button twice and the sample is corrected again. I open all samples in 5.7 first and then in 7.6 version to avoid autocopmensation that FlowJo incroporate without asking me. Afterwards, I analyse the samples in 7.6 without problems
Hey Enrique,
This is due to there being at least one parameter in the FCS file loaded that is present in the matrix. You might try and check those parameters, if they are all there, then send me your analysis saved as an ACS file to [email protected] or here for closer examination. Also, you might try and make sure your files are connected to the workspace, ie. have not changed name or location/server disconnect.
Best regards,
Miguel
FlowJo Tech Support
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