Hi,
I want to prepare Tris-Triton lysis buffer that contains 1mM EDTA. Of course I have problems with dissolving EDTA i Tris solution (pH= 7.4). I got a tip to dissolve EDTA at pH close to 8. So, my question is: Do you prepare any stock EDTA solutions? Is pH of this solution =8? If I would add this stock solution with pH=8 to the other components of the lysis buffer will the pH of this buffer be ok and will it work? Or maybe you prepare more stock solutions for this buffer?
Please, help me to find an easy way to mix it! :)
EDTA is an acid that can bind up to 4 cations like Na+. This increases the pH. I always have a 1 M stock solution handy, prepared by adding just enough NaOH that the EDTA goes into solution. When you dilute this 1000-fold, the effect on the pH of your buffer will be minimal.
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Biochemical Techniques
- Analytical Chemistry
- pH