ANCOVA controlling baseline in RCT - how to perform power analysis and sample size estimation?

Hi, I have a simple question. I am hoping to perform a power analysis/sample size estimation for an RCT. We will be controlling for baseline symptoms, and using post-treatment or change scores as...

19 February 2021 2,648 23 View

Why am I getting western bands with white bubbles?

12 October 2020 6,976 3 View

Ion torrent - What happens if the amplicons are too long?

Hello everyone, What happens if I try to sequence with Iron torrent amplicons (eg of a marker gene) that are too long? By that I mean more than 500bp. We did try (not on purpose, we did not...

11 August 2020 368 3 View

How would I determine if a protein (PD-1) is necessary for establishment of latent infection by a virus in a CD4 memory cell line?

If I were to plan an experiment to deduce whether or not PD-1 is absolutely necessary for HIV to establish latent infection in CD4 memory cells, how would I go about doing this? More...

09 March 2020 7,774 2 View

Can we unbias data from umbrella simulations with missing data points?

Imagine a system with 2 energetic basins A and B separated by a high barrier. We cannot sample that barrier for whatever reason (ie. because the lowest energy path is very "long" with lots of...

11 September 2019 9,156 1 View

What is the most appropriate statistical analysis to assess between and within subject differences over time with most individuals with 2 time points?

I am looking at how hormone concentrations change over time both between individuals and in relation to fixed effects of predictor variables (n=7) within individuals. I have a large dataset of...

02 July 2019 8,734 9 View

I want to selecte resistance antibiotic bateria in the pond ? I was offer the MHA already but Could you give me some idea about an other medium ?

The antibiotic target is SMX and OCT,

30 June 2019 5,542 5 View

Calculating beam nodal displacements/rotations using SAP2000 and programming system matrices in MATLAB - Differences in responses?

I have programmed an Euler-Bernoulli beam, supported on springs, using both SAP2000, and by using the stiffness matrix method in MATLAB. The nodal (joint) displacements calculated due to an...

13 March 2019 8,597 3 View

Can you recommend any peptide-coated microplates?

Hi! Can you recommend any company (or at least a research-team) where I can order a microplate covered with peptides according to my needs? I need it for immunological assays. Thanks for all...

12 February 2019 5,959 2 View

Can you recommend any peptide-coated microplates?

Hi! Can you recommend any company (or at least a research-team) where I can order a microplate covered with peptides according to my needs? I need it for immunological assays. Thanks for all...

01 February 2019 9,590 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View