Karolina Nowicka-Bauer

6 Questions 28 Answers 0 Followers

Questions related from Karolina Nowicka-Bauer

Can you recommend any peptide-coated microplates?

Hi! Can you recommend any company (or at least a research-team) where I can order a microplate covered with peptides according to my needs? I need it for immunological assays. Thanks for all...

02 February 2019 9,722 2 View

Do you know any good company producing commercially nanoparticles?

Hi! Can anyone of you recommend a company that synthesize good nanoparticles? Ideally, if we could by already loaded nanoparticles with an agent of our interest. Will appreciate all of your...

08 August 2018 10,041 6 View

Tris-Triton Lysis Buffer - any tips how to dissolve EDTA?

Hi, I want to prepare Tris-Triton lysis buffer that contains 1mM EDTA. Of course I have problems with dissolving EDTA i Tris solution (pH= 7.4). I got a tip to dissolve EDTA at pH close to 8. So,...

02 February 2018 3,083 1 View

"Clouds" in 8.8 pH Trizma-HCl buffer?

Hi, I have found my Trizma-HCl buffer (strored in a fridge) to be contaminated with sth like "clouds" (see pictures). I prepared a fresh solution and during sdjusting the pH with NaOH (40% or...

01 January 2018 6,446 3 View

In 1D Western blot, why do I have that smear effect and some bands are white in their centers?

I have already reduced the concentration of primary antibody and incubation time with ECL. It worked but my 3 last blots look like that again. Any suggestions? photo in the attachment

11 November 2014 7,731 4 View

Is there any reference protein (like beta-actin) for (human) seminal plasma?

Like in the headline. We have found a publication with PAP (prostatic acid phosphatase) but it seems that it doesn't work - too many differences between individuals. I want to compare the...

10 October 2014 956 7 View