Hello. I am using Miltenyi's Magnetic Treg isolation (CD4+CD25+CD127 dim).
Does any one have experience if I can use the LS column for the 1st isolation, instead of the LD depletion columns?
What are the differences in yield? in purity?
I would recommend using the LD column.
My experience with LS comun is that the purity is really good but if you want to isolate further cells from the suspension which already passed by the magnet and was not retended, you ll end up with a lot of cells from your primary magnetic labeling.
For example, I tried to isolate CD4+ and CD8+ T cells from PBMC.
So I started isolating CD8+ cells and labeled all unbound cells with CD4+microbeats for a second column run. It happens that your CD4+ fraction contains as much as 20% of CD8% T cells (no other cells, so it is due to the magnetic labeling).
Hope this helps. Greetings