How can one perform the Hadri and Rao (2008) panel unit root test with structural break in STATA or R?

Hi, I am looking forward to test unit root for a panel data series. In this regard, I would want to use the Hadri and Rao (2008) test with structural break. Is there any way, I can perform the...

29 September 2019 2,667 2 View

How to measure ROS of sperm (isolated from rat) by H2DCFDA via flow cytometry?

Hello, I am interested to perform ROS measurement from isolated rat's sperm by flow cytometry by H2DCFDA. So, is there any standard protocol which I need to follow to get the results along with...

04 May 2018 2,660 5 View

Does miscibility in water differ for surfactant solutions (cationic, anionic and nonionic) ?

three solutions are of cationic, anionic and nonionic surfactant. are there differences in miscibility properties.

01 May 2016 1,931 5 View

What libraries would make Java easy to use for scientific computing?

So a friend and I will probably get into a project where we try to make mathematical and scientific libraries for Java, possibly in native code. So a few quick questions that come to mind...

12 March 2015 4,392 11 View

Are there any fluorescent materials for a violet/blue to red conversion?

I've been looking into fluorescence, and I see a lot of work on the phosphor that we normally talk about, in CFLS, tube lights, etc, but I don't see any optical-to-optical conversion fluorescence...

23 February 2015 6,261 7 View

How can I assign positive or negative charges on a specific atom in a molecule?

31 July 2014 8,219 10 View

Can anyone help me find out the MO composition in a metal complex?

How can I find out the % of metal character and % of ligand character in a particular MO of a metal complex?I have done a few NBO calculation but It only gives the bonding composition.

27 June 2014 4,622 12 View

What are the pros and cons of using a LiNBO3 waveguide core over Si waveguide core?

From fabrication point of view as well as application point of view?

26 October 2013 3,076 2 View

What are the components of the hydrogel? Is it fully synthetic?

I am interested in the chemical aspect of the hydrogel being used so asked the above mentioned question

01 January 1970 6,133 2 View

Ultra structural analysis of testicular tissue by Scanning Electron Microscopy?

Hello, I am interested to perform SEM on rat testicular tissue as well as on isolated sperm. So, is there any standard protocol which I need to follow to get the results along with sample...

01 January 1970 2,403 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

How are wild-type and mutant gene and protein isolations and analysis performed?

Considering that we hypothetically designed an experiment, we have an x gene, and we have the mutant version of this gene with a single nucleotide mutation in one of the exons. What kind of an...

22 January 2021 9,589 12 View

How can I prepare 10mM sodium acetate buffer with pH 9?

I have found an article (Extraction, physicochemical characteristics and functional properties of Mung bean protein) for extracting mung bean protein isolate using water as solvent. As my...

21 January 2021 8,995 3 View

Anybody to help me with Code source for DOUBLE CONCAVE FRICTION PENDULUM BEARINGS Please?

Hello, would you please share the codes for Base Isolation of structures using Double Concave Friction Pendulum Bearings? I am doing a related research but as I am just a beginner in MATLAB, I...

18 January 2021 6,683 1 View

How to extract protein after trizol RNA and back extraction of genomic DNA?

I have limited amount of samples, so I am trying to get RNA, DNA and protein from the same samples. I used Trizol reagent for RNA isolation, then use back extraction buffer for genomic DNA...

13 January 2021 1,566 2 View

Culturing Peziza sp?

Hi everyone, Any successful trials for getting some cultures from Peziza Fruitbodies? We have done several trials using usual medium of PDA/ MA, but all failed. Thanks in advance

29 December 2020 6,966 6 View

Cleaning up single cell RNA from 10x dataset?

Hello, I am new to single cell RNA data processing and am currently running my data through the initial cleanup steps via Seurat on R. I have previously done this on epithelial organoid data and...

23 December 2020 4,709 1 View