Sayani Haldar

6 Questions 3 Answers 0 Followers

Questions related from Sayani Haldar

Could someone please share the procedure for preparing cell lysates from the HepG2 cell line?

I am preparing protein lysate from the HepG2 cell line using RIPA buffer containing NP-40. After centrifugation, three layers are formed: an upper lipid (fat) layer, a middle clear layer, and a...

18 June 2025 6,584 1 View

Query regarding subcloningHow many nucleotides can be added to the 5' side of the forward primer, aside from the restriction enzyme overhang?

I'm planning to perform subcloning and need to include restriction enzyme sites in the primers to amplify the insert. In addition to the restriction enzyme overhang on the forward primer, I also...

18 February 2025 3,911 2 View

Can we distinguish 4733 bp plasmid from 6248 bp plasmid in the 1 percent agarose gel?

Can we distinguish 4733 bp plasmid from 6248 bp plasmid in the 1 percent agarose gel? Can anybody help me with their suggestions?

10 November 2024 5,626 3 View

To do gibson assembly cloning can I use DH5alpha strain of E. coli. for transformation?

21 June 2024 826 1 View

Why I am not getting colony after transformation in Dh5alpha?

I am trying to do gibson assembly cloning. I have two fragments for gibson assembly cloning (plasmid backbone and insert). After gibson assembly reaction I have transformed this in E. coli....

20 June 2024 6,461 4 View

Issues regarding gibson assembly

I am trying to do Gibson assembly. After ligating insert with backbone I have transformed the ligated plasmid in DH5alpha via electroporation. After transformation I got some colonies in Luria...

01 January 1970 2,005 3 View