Hello,
Our lab has started working with the enteroid derived from the fetal intestinal tissue. I have 2 challenging queries as follows-
1. For mincing the intestine, so far, I have been using a scalpel but still, the mincing is not fine and smooth, rather I get fragmented tissue that results in unsuccessful cell line establishment. How can I mince the biopsied intestine very well to get a single cell line from that?
2. How can I prove that my working tissue is from the intestine i.e., is there any specific biomarker I can detect that will help to prove that the enteroid established from the biopsied tissue is actually fetal intestinal tissue?
TIA.
To generate single cells, you can digest the tissue with Trypsin or Collagenase after mince. You may need to test out the digest time for your tissue.
Thank you, Xiuquan Luo. I will look into more detail on how it ging to work for fetal intestinal tissue biopsy.
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