Hello,

Our lab has started working with the enteroid derived from the fetal intestinal tissue. I have 2 challenging queries as follows-

1. For mincing the intestine, so far, I have been using a scalpel but still, the mincing is not fine and smooth, rather I get fragmented tissue that results in unsuccessful cell line establishment. How can I mince the biopsied intestine very well to get a single cell line from that?

2. How can I prove that my working tissue is from the intestine i.e., is there any specific biomarker I can detect that will help to prove that the enteroid established from the biopsied tissue is actually fetal intestinal tissue?

TIA.