Help with your experience and suggestion, please!!!!
We are working with human milk sample i.e., post partum women's milk. We are planning to see the microbiome variation in the milk. I did couples of literature review and exactly followed the described protocol over there to extract the DNA from the postpartum womens milk. But nothing is working perfectly. Our Qubit and Nanodrop measure DNA concentration is so low and 260/280 ratios are so varied sample to sample that I am wondering what happened with my milk samples. That's disappointing.
Why I am not getting good DNA with good 260/280 ratios!!
I will appreciate your suggestion, idea, protocol etc.
Thank you.
WHAT is the age of the mother , are you talking the colostrum in the first three days.
also try to review the procedure and the portocole.
usual DNA mainly early in other milk and disappear after 20 months completely.
Thank you so much, Aziz Koleilat for your response.
We do collect total 3 samples from 1 mother.
1 in between 1 and 2 months
1 in between 3 and 4 months
1 in between 5 and 6 months.
In fact, the amount of DNA in milk is almost nonexistent
Their source is epithelial tissue.
- Badges
- Science topic
- Similar topics
- Phytochemistry
- Extracts