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Questions related from Shaheda Urmi
Hello everyone, Have anyone of you ever extracted both DNA and RNA from the PBMC sample stored in Qiazol!!! I know that we only can extract RNA from Qiazol but I also need the DNA from that...
04 October 2019 6,481 6 View
Help with your experience and suggestion, please!!!! We are working with human milk sample i.e., post partum women's milk. We are planning to see the microbiome variation in the milk. I did...
01 October 2019 7,976 3 View
Hello, Our lab has started working with the enteroid derived from the fetal intestinal tissue. I have 2 challenging queries as follows- 1. For mincing the intestine, so far, I have been using a...
01 January 1970 2,654 2 View
Hi everyone, I have to do postpartum milk DNA extraction for my project using QIAGEN kit. I use Qubit flurometer and also the Nano to measure the DNA concentration. Matter of thing is, don't know...
01 January 1970 7,279 4 View
Hello everyone, I have to separate monocyte from the PBMC for my project. I use ficoll gradient method to separate the buffy coat and then use MACS cell separator and flow cytometer to quantify...
01 January 1970 475 6 View
Hello, For my enteroids establishment, I have been using scalpel to mince fetal intestine but still te tissue remain fragmented rather than minced. Do you have any better techniques for...
01 January 1970 5,350 0 View
