Is it possible to use the negatively stained carbon grid again for TEM imaging of viruses?

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How to remove bubbles during preparation of agarose gel or after prepared it?

1. How to remove bubbles during preparation of agarose gel or after prepared it? 2. When we inject the gel inside the capillary tube, there are some bubbles visible. What is the way to...

22 September 2023 3,951 0 View

How to culture neuron cells preferably motor neurone cells to test if the Antisense oligonucleotide based drug is working?

I am working on Spinal muscular atrophy (SMA). My target is to develop an Antisense Oligonucleotide (ASO) based drug for the treatment of this disease. For this, I want to see if the ASO I am...

16 July 2023 5,360 1 View

Can anyone suggest how I get the matrix of IBR values?

Score number 9, calculate 9 Ai for each group. What is the maximum number of IBR values I can calculate with score 9 and how can I get the Matrix for IBR values?

09 June 2023 6,190 0 View

How to do plaque assay for H1N1?

I am trying to establish a plaque assay for the H1N1 strain but can not make it after multiple tries. MDCK cells were used. Media I used for H1N1 growth and plaque assay stated below (for...

26 February 2023 8,717 2 View

How to calaculate Statistical mono window (SMW) algorithm coefficients for LST?

I'm attempting to understand how the statistical mono window (SMW) algorithm coefficients are computed but it is unclear. I am following Sofia Ermida's paper but facing difficulties in...

19 December 2022 9,083 1 View

How mask Cloud from ASTER - AST_05 collection (L2 surface emissivity) and collection AST_07 (L2 surface Reflectance)?

It's been a while I am trying to find out cloud masking method from ASTER images L2 products. Is there any method? Code?

10 October 2022 2,108 0 View

How to mask cloud in Google earth engine without transperant area after masking?

I am trying to do LST analysis using GEE. But when the cloud is removed, a significant area is transparent with no value. That impacts the result of my analysis. I would appreciate you if anyone...

29 May 2022 2,880 3 View

Is this possible that if I didn't get any high molecular weight protein band in Ponceau will give result after Ab incubation and colour development?

protein molecular weight above 260 kDa liver sample abundant protein equal loading, validated through housekeeping.

02 March 2022 5,287 0 View

How to measure water temperature (stream) using remote sensing?

There are method for monitoring sea surface temperature. But How can I measure for inland stream, river temperature?

26 January 2022 6,575 12 View

Is Kruskal Wallis test important to perform even after performing one-way Anova where we got significant difference between groups?

In my statistical analysis alpha value is less than 0.05. I have performed one way ANOVA and Tukey post hoc test where I got significance wherever it is applicable. But to understand the...

12 September 2021 9,586 6 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Smart grid ideas?

How to use and this is economical?

06 August 2024 3,160 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View