03 March 2022 0 5K Report

protein molecular weight above 260 kDa

liver sample

abundant protein

equal loading, validated through housekeeping.

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How to convert 2D stack images to 3D to obtain surface area of nucleus?

I have 15 slices of the nucleus at different z-heights from confocal imaging. I would like to use these slices to reconstruct the 3D nucleus and ultimately estimate the surface area of the...

20 February 2021 6,967 1 View

I want to convert Methane gas to liquid Methanol using photocatalysis. Can you please suggest me a good paper which I can study ?

There are a number of publications regarding this, but none of them are clear and show the mechanism of gaseous Methane conversion to liquid Methanol.

06 January 2021 364 3 View

Can wavelet packet sub-bands be stacked together to form an image?

I have a 1D signal and I have done wavelet packet decomposition on it which is giving several sub-bands. Can I stack these sub-bands (one below other) to form a 2D matrix and hence an image...

27 November 2020 1,269 5 View

How can I maintain a surface at isothermal condition, say at 300 deg Celsius.?

I have 6 heaters each of power 0.5 kW and mounted below a curved mild steel sheet of thickness 4 mm. The heaters and the below section is covered and insulated. I want the top surface to be...

05 November 2020 199 5 View

what are the possible reasons for Crop Water Stress Index values higher than 1 under moisture stress condition?

I have determined CWSI using the theoretical approach of Jackson et al., (1982) based on canopy temperature measurement in chickpea. at the final stages of crop growth some of my values of CWSI...

28 October 2020 9,992 3 View

Do always a Good paper depends on How much Citations it Received. I have seen many Good Research Papers with zero or less Than 10 Citations?

Its a Research Paper related Question

22 October 2020 6,179 4 View

Help in cloning, should I create 5' phosphate group on digested vector?

Hello all, I am trying to introduce an annealed DNA fragment into the double digested vector. The ssDNAs were ordered without a phosphate group modification. The vector is digested with XhoI and...

20 October 2020 8,357 5 View

Can anyone explain the calculation process of integrated biomarker response (IBR) used in chronic toxicity study with an arbitrary dataset?

Video tutorial on IBR calculation or stepwise procedure..

05 October 2020 9,279 3 View

Could someone provide the permeability value for 2% Chromium doped BiFeO3?

Any close value will also work.

03 October 2020 8,067 4 View

Purification of annealed oligonucleotides after annealing procedure?

Hello all, I will do oligonucleotides annealing for the first time which will be later used for cloning without any more modification. Can any of you kindly suggest if I should purify the annealed...

16 September 2020 3,881 3 View

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fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

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