Dear knowledgeable,
Have been doing neuronal cell cultures for many years now, (yikes). I've found it useful to "balance" my media in the incubator prior to applying it to the cells. This meaning approaching equilibrium of temperature (37dC) and CO2 between the atmosphere in the incubator and the media. Especially with Bicarbonate media this keeps pH in-check.
Have no exact sources but usually about 30minutes.
I recently started doing low oxygen cultures, where O2 is kept at around 5% (by flooding with nitrogen). I am not sure of the details of how much N2 is actually used to balance out the 02.
My question is therefore, how long time should I leave my my norm-ox (~20%) media in the incubator to reach low-ox (5%) equilibrium.
Thinking one of you clever chemists might be able to give an approximation?
Friendly regards, Staffan
Ps. For this example. Imaging a filled 50ml tube - Surface area ~5-7.5cm2, and the surface undisturbed ;)
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