Hi guys,
I'm trying to look at the LDH activity in primary neurons, however we dont have much money to spend on assays kits... so, anyone have an idea to look at the enzyme activity without a specific kit?
Thanks!
Hello Luiz Felipe Souza e Silva
You can have a look at the protocol mentioned in the research paper below.
THE ENZYMATIC ASSAY OF PYRUVIC AND LACTIC ACIDS. A DEFINITIVE PROCEDURE
Clin. Chim. Acta, 19 (1968) 357-361
DOI: 10.1016/0009-8981(68)90258-1
PMID: 4296123
I hope the protocol is helpful.
Good Luck
Hello Malcolm,
In this paper they dont have a specificy method to the LDH mesurament. But thanks!
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