Luiz Felipe Souza e Silva

5 Questions 5 Answers 0 Followers

Questions related from Luiz Felipe Souza e Silva

IPS cells - blackdots - help?

I'm working with iPS cells, and I know that some researchers here have been facing the same problem as I am: black dots appearing on the adherence matrix (Geltrex, in my case). Every time I do a...

27 January 2025 7,634 2 View

Mitosox in mitochondrial extract?

Hi everyone, I was wondering if someone has a protocol for using MitoSOX in mitochondrial-enriched extracts. MitoSOX can migrate to the nucleus and generate an inaccurate response about...

25 March 2024 508 0 View

How to do imunofluorescence in neurosphres?

Hi! I'm doing neurospheres from neonates. I have then in suspension, and I need to do immunofluorescence. I always look at beautiful pictures from google images and the neurospheres are in...

29 June 2022 5,524 0 View

Co-culture - Neurons and astrocytes?

Hi guys! I'm trying to co-culture neurons and astrocytes. I'm using a protocol that first grows the neurons into plates (let then grow) and then I put in the astrocytes (fully grown) and...

07 September 2021 4,425 3 View

There is a way to mesuare LDH activity without a kit?

Hi guys, I'm trying to look at the LDH activity in primary neurons, however we dont have much money to spend on assays kits... so, anyone have an idea to look at the enzyme activity without a...

04 June 2021 5,834 2 View