Hello everyone,
In my upcoming project, I will use poly-L-lysine coated culture flasks due to the culture conditions of neural stem cells.
One thing that comes to my mind is if I need to perform a viability test such as MTT or SRB on these cells, do the 96-well plates have to be poly-L-lysine coated as well? Buying manufactured coated plates increases the cost a lot. I am guessing my deviations may increase if I coat them myself. Also, does poly-L-lysine interfere with these kinds of tests? I do not have much experience with this subject. I would be very happy if you share your information not only for the viability tests but also for any kind of plate-based tests for the neural stem cells.
Thank you very much.