16 Questions 36 Answers 0 Followers
Questions related from Gülşah Torkay
Hello everyone, We needed cardiac cells for a project and obtained H9C2 rat cardiovascular myoblast cells from another laboratory. The cells are at passage 15 and unfortunately have...
08 October 2023 7,557 1 View
I will use retinoic acid (Sigma/R2625) and as I know is sensitive to air and light. After dissolving the chemical, I want to store my aliquots in an inert environment, but I do not have suitable...
19 September 2023 6,674 3 View
Hello everyone, This is my first time working with a neural stem cell line. The cells reached me in passage 1. I use PLO and laminin-coated plates for culturing. At the first passage, cells did...
24 March 2023 9,125 3 View
Hello everyone, To work with a neural cell type, I will cover the flasks and dishes I use with poly-l-ornithine. I have a Sigma product with catalog number P3655. In the datasheet, I was unable...
14 March 2023 4,377 3 View
Hello everyone, In my upcoming project, I will use poly-L-lysine coated culture flasks due to the culture conditions of neural stem cells. One thing that comes to my mind is if I need to perform...
24 October 2021 1,367 3 View
Hello everyone, I conducted a series of experiments in which I compared the efficacy of an anticancer agent with different viability tests. I repeated five wells for all drug doses in each test....
21 August 2021 8,118 3 View
Hello, I will develop materials for the repair of nerve damages in my master thesis, and I am planning to use neural cells for cell culture experiments. I have cell culture knowledge, but I have...
02 July 2021 5,521 4 View
Considering that we hypothetically designed an experiment, we have an x gene, and we have the mutant version of this gene with a single nucleotide mutation in one of the exons. What kind of an...
23 January 2021 9,852 12 View
Hello everyone, I'm having some trouble with IC50 dose calculations. I try to do differently in Prism, Excel, etc. and the results are not matching sometimes. And sometimes my dose-time plots do...
15 April 2020 8,362 4 View
Hello everyone, I have never seen it before but I wonder if that is possible or not? Can I calculate IC50 value from DNA fluorescence? Thanks for your help!
08 February 2020 1,408 4 View
Hi everyone, I am working on an experiment that requires nearly one-week incubation on 96-well plates. In this experiment, I am also measuring "time zero" to understand the cells' condition after...
30 January 2020 9,941 5 View
Hello everyone, As I wanted to see an agent's activity on my cells, I have measured DNA fluorescence via Hoechst dye. This agent prevents chain elongation by incorporating into DNA, afterward...
08 January 2020 1,274 7 View
Could you please suggest me some papers, textbooks and basic tools for learning in silico methods? Primarily, I want to research about cancer metabolism, drug resistance, and epigenetics. For...
28 November 2019 628 2 View
Do you know any database for searching about endemic species, their genomes, differences, DNA barcodes etc.?
28 November 2019 284 3 View
Hello everyone, I want to see HMGB1 release to cell culture media to detect the necrosis levels. For this reason, I have used both lysates and cell culture media to make Western Blotting. But my...
22 August 2019 7,253 11 View
Hello everyone, I need exosome free serum for my cell culture media. I`d tried to use Beckman Coulter - Optima L-100 XP at 100k g but my tubes (Thinwall, Polyallomer, 94 mL, 38 x 102 mm) got...
06 August 2019 5,882 18 View
