Gülşah Torkay

16 Questions 36 Answers 0 Followers

Questions related from Gülşah Torkay

The H9C2 cardiomyoblast cells differentiated into myotubes. Is there anything we can do about this?

Hello everyone, We needed cardiac cells for a project and obtained H9C2 rat cardiovascular myoblast cells from another laboratory. The cells are at passage 15 and unfortunately have...

08 October 2023 7,551 1 View

How can I easily keep my chemical aliquots inert?

I will use retinoic acid (Sigma/R2625) and as I know is sensitive to air and light. After dissolving the chemical, I want to store my aliquots in an inert environment, but I do not have suitable...

19 September 2023 6,669 3 View

Can I use TrypLE instead of accutase in the rat neural stem cell?

Hello everyone, This is my first time working with a neural stem cell line. The cells reached me in passage 1. I use PLO and laminin-coated plates for culturing. At the first passage, cells did...

24 March 2023 9,122 3 View

Can I store my poly-l-ornithine stock by making aliquots?

Hello everyone, To work with a neural cell type, I will cover the flasks and dishes I use with poly-l-ornithine. I have a Sigma product with catalog number P3655. In the datasheet, I was unable...

14 March 2023 4,374 3 View

Do I need to use a poly-L-lysine coated plates for assays on neural stem cells?

Hello everyone, In my upcoming project, I will use poly-L-lysine coated culture flasks due to the culture conditions of neural stem cells. One thing that comes to my mind is if I need to perform...

24 October 2021 1,365 3 View

Why are the standard deviations of my experiments under the same conditions so different?

Hello everyone, I conducted a series of experiments in which I compared the efficacy of an anticancer agent with different viability tests. I repeated five wells for all drug doses in each test....

21 August 2021 8,114 3 View

What is your recommendation for neuronal cultures?

Hello, I will develop materials for the repair of nerve damages in my master thesis, and I am planning to use neural cells for cell culture experiments. I have cell culture knowledge, but I have...

02 July 2021 5,517 4 View

How are wild-type and mutant gene and protein isolations and analysis performed?

Considering that we hypothetically designed an experiment, we have an x gene, and we have the mutant version of this gene with a single nucleotide mutation in one of the exons. What kind of an...

23 January 2021 9,841 12 View

Is there any easy way for the IC50 calculation?

Hello everyone, I'm having some trouble with IC50 dose calculations. I try to do differently in Prism, Excel, etc. and the results are not matching sometimes. And sometimes my dose-time plots do...

15 April 2020 8,359 4 View

Is it possible to calculate IC50 value from DNA fluorescence?

Hello everyone, I have never seen it before but I wonder if that is possible or not? Can I calculate IC50 value from DNA fluorescence? Thanks for your help!

08 February 2020 1,400 4 View

Why do my control cells begin to die on day one?

Hi everyone, I am working on an experiment that requires nearly one-week incubation on 96-well plates. In this experiment, I am also measuring "time zero" to understand the cells' condition after...

30 January 2020 9,933 5 View

Can I perform Bradford on the plates which I measure DNA fluorescence before?

Hello everyone, As I wanted to see an agent's activity on my cells, I have measured DNA fluorescence via Hoechst dye. This agent prevents chain elongation by incorporating into DNA, afterward...

08 January 2020 1,268 7 View

How can I improve myself in "in silico" experiments and models?

Could you please suggest me some papers, textbooks and basic tools for learning in silico methods? Primarily, I want to research about cancer metabolism, drug resistance, and epigenetics. For...

28 November 2019 623 2 View

Is there any database for endemic species?

Do you know any database for searching about endemic species, their genomes, differences, DNA barcodes etc.?

28 November 2019 278 3 View

What is the problem about my Western Blot bands which I have obtained to detect HMGB1 release in cell culture media?

Hello everyone, I want to see HMGB1 release to cell culture media to detect the necrosis levels. For this reason, I have used both lysates and cell culture media to make Western Blotting. But my...

22 August 2019 7,246 11 View

Could you inform about obtaining an exosome depleted FBS with ultracentrifuge?

Hello everyone, I need exosome free serum for my cell culture media. I`d tried to use Beckman Coulter - Optima L-100 XP at 100k g but my tubes (Thinwall, Polyallomer, 94 mL, 38 x 102 mm) got...

06 August 2019 5,880 18 View