How are wild-type and mutant gene and protein isolations and analysis performed?

Considering that we hypothetically designed an experiment, we have an x gene, and we have the mutant version of this gene with a single nucleotide mutation in one of the exons. What kind of an...

22 January 2021 9,589 12 View

Can't the Python sklearn decision tree classifier correctly handle categorical attributes?

Hello, I'm trying to model my dataset with decision trees in Python. I have 15 categorical and 8 numerical attributes. Since I can't introduce the strings to the classifier, I applied one-hot...

24 July 2020 5,787 3 View

How can I apply position restraint to a bilayer during Umbrella sampling for PMF calculations?

Hi all, I have a couple of questions for PMF calculations in Gromacs. I placed a nanoparticle (NP) in the water region approximately 1nm away from the membrane and by using Gromacs pull code, I...

21 April 2020 3,827 4 View

Is it possible to calculate IC50 value from DNA fluorescence?

Hello everyone, I have never seen it before but I wonder if that is possible or not? Can I calculate IC50 value from DNA fluorescence? Thanks for your help!

07 February 2020 1,302 4 View

Why do my control cells begin to die on day one?

Hi everyone, I am working on an experiment that requires nearly one-week incubation on 96-well plates. In this experiment, I am also measuring "time zero" to understand the cells' condition after...

29 January 2020 9,826 5 View

Can I perform Bradford on the plates which I measure DNA fluorescence before?

Hello everyone, As I wanted to see an agent's activity on my cells, I have measured DNA fluorescence via Hoechst dye. This agent prevents chain elongation by incorporating into DNA, afterward...

07 January 2020 1,167 7 View

How can I improve myself in "in silico" experiments and models?

Could you please suggest me some papers, textbooks and basic tools for learning in silico methods? Primarily, I want to research about cancer metabolism, drug resistance, and epigenetics. For...

27 November 2019 531 2 View

What is the problem about my Western Blot bands which I have obtained to detect HMGB1 release in cell culture media?

Hello everyone, I want to see HMGB1 release to cell culture media to detect the necrosis levels. For this reason, I have used both lysates and cell culture media to make Western Blotting. But my...

21 August 2019 7,158 11 View

How do I calculate bilayer thickness with Gromacs distance command?

Hello all, I carried out a molecular dynamics simulation for a coarse grained membrane system. I want to select the PO4 groups in top and bottom leaflets and calculate the vertical distance. How...

16 June 2019 9,348 4 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View