Hello,
I just started working on Exosomes-ELISA research. I found out that people (who are working in this filed) are using different compositions of Assay and Washing buffers for their work.
The main objective of my request here is to know the right compositions of Assay and Washing buffers for capturing & detecting exosomes using antiCD9. Could you please share your recipes here on this board?. Any help regarding this would be greatly appreciated.
Thank you very much in advance.
Hello Venkata Suresh Reddy Vajrala , we used all wash buffers Elisa kit itself. You are using kit of some company ?
Awaiting,
Marina
Hello Marina ...
No I am not. I have decided to start with (PBS + 0.5%BSA + 0.05% Tween 20) and superblock buffer.
fingers crossed
Thanks for your reply
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