Targets of transcription factor

More Jana Pb's questions See All

Can anyone suggest where I can get wolbachia bacteria?

I'm working on wolbachia bacteria to study it's antiviral activities in silk warm cell lines. However, I'm not finding the source of bacteria to purchase. Can anyone help me regarding this?

10 November 2015 8,649 3 View

Can someone shed some light on the difference between tumor sphere and spheroid ?

Any difference between tumor sphere and spheroid ?

06 July 2013 3,458 2 View

What is the best method to get neuronal cells from Medulloblastoma Neurospheres?

I used all trans retinoic acid to differentiate neurospheres generated from medulloblastoma cell lines. I observed neuronal cells but not in sufficient amounts. Can anyone suggest how I can get a...

06 July 2012 5,300 3 View

hi

Any could suggest to the right protocol for the differentiation of Neurospheres. Thank you, jana.

02 March 2010 6,946 1 View

protocol

hi, if any one have protocol for Immunocytochemistry/immunoflourescence ...to identify CD133 in neurospheres derived from DAOY cell lines... jana.

31 December 2009 6,356 1 View

Hi

Hi, I am jana from Liverpool UK, doing my masters... my research project is on..brain cancer and dedifferentiation...i am trying to identify CD133 positive cells in DAOY medulloblastoma cell...

31 December 2009 8,639 0 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

Shupeng Liu

u can first predict the targets using softwares or databases, then ensure the results by CHIP.

Jana Pb

Thanks but I need the names of the databases. please any one could suggest.

jana.

http://mapper.chip.org/ this is one of them. u can search online for others.

Lisardo Ugidos

What type of cance? The optimal markers change from one type of cancer to other.