Can anyone suggest where I can get wolbachia bacteria?

10 November 2015 8,582 3 View

What is the best method to get neuronal cells from Medulloblastoma Neurospheres?

I used all trans retinoic acid to differentiate neurospheres generated from medulloblastoma cell lines. I observed neuronal cells but not in sufficient amounts. Can anyone suggest how I can get a...

06 July 2012 5,231 3 View

Targets of transcription factor

Hello, Any one could inform me how to find targets of transcription factor TOX3. I need to know whether TOX3 can target stem cells genes such as OCT3/4, NANOG, SOX2, c-MYC, CD133 and also...

10 November 2010 3,546 4 View

hi

Any could suggest to the right protocol for the differentiation of Neurospheres. Thank you, jana.

02 March 2010 6,857 1 View

protocol

hi, if any one have protocol for Immunocytochemistry/immunoflourescence ...to identify CD133 in neurospheres derived from DAOY cell lines... jana.

31 December 2009 6,297 1 View

Hi

Hi, I am jana from Liverpool UK, doing my masters... my research project is on..brain cancer and dedifferentiation...i am trying to identify CD133 positive cells in DAOY medulloblastoma cell...

31 December 2009 8,566 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View