Suitable DNA codon for the linker (G4S)3 for expression in E. coli?

Desmond Omane Acheampong @Desmond_Acheampong

03 March 2013 1 8K Report

I want to link two proteins with the linker (G4S)3. The linked (fused) proteins will then be expressed in E. coli. I will therefore be grateful if anybody could recommend a suitable DNA codon for this linker.

Amin Tashakor

Just check the bacterial codon usage and select those with the most frequency in bacteria.

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