I want to fuse MIC-A and ScFv with a linker, but I am confused about the construction. Please can you help? Should it be MICA-ScFv or ScFv-MICA?

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Mark Sutherland

If there are no other fusion proteins of MIC-A you may need to try both as the fusion to either amino or carboxy end may result in loss of activity. If someone has created a different fusion (e.g MICA-GFP or GFP-MICA) I would use the same orientation that they used as it would suggest activity is retained. As far as I know I don't think the scFv should be influenced by the orientation.