I want to express these fused proteins and need to know which of the proteins should come first. This is to guide me in the primer design. MIC-A is a ligand of NKG2D and ScFv is a single chain variable fragment (antibody fragments).
If there are no other fusion proteins of MIC-A you may need to try both as the fusion to either amino or carboxy end may result in loss of activity. If someone has created a different fusion (e.g MICA-GFP or GFP-MICA) I would use the same orientation that they used as it would suggest activity is retained. As far as I know I don't think the scFv should be influenced by the orientation.