Desmond Omane Acheampong

7 Questions 24 Answers 0 Followers

Questions related from Desmond Omane Acheampong

What is the best way to test the binding affinity of a Ligand (MICA) and its receptor (NKG2D) expressed in E coli?

MICA and its receptor NKG2D were expressed as an inclusion bodies in E coli. It was therefore denatured and renatured employing dialysis method of refolding. Carrying out ELISA of MICA and it...

01 January 2014 7,648 7 View

What pH of refolding buffer will be suitable for refolding (renaturing) a solubilized Inclusion Body of protein with isoelectric point (PI) 6.2?

I am trying to solubilize and refold an inclusion body of NKG2D with theoretical isoelectric point (PI) value of 6.2. I usually purify my solubilized protein with Nickel column (Elution buffer pH...

12 December 2013 9,857 5 View

Can any one help me with a suitable protocol for double digestion of a vector(pET22b) and an insert (DNA) with BAHM1 and NOT1 restriction enzymes?

I want to insert my recombinant DNA of about 1650bp into pET22b which is about 5500bp. The resultant product from the ligation will be used for E. coli transformation. I seem not to get a...

09 September 2013 10,028 12 View

Which cell line is the most suitable for the expression of the NKG2D receptor?

NKG2D is a receptor expressed on NK and T cells. I would be grateful if anybody could suggest a suitable cell line that can express NKG2D on its surface for in vitro studies.

03 March 2013 8,054 0 View

Suitable DNA codon for the linker (G4S)3 for expression in E. coli?

I want to link two proteins with the linker (G4S)3. The linked (fused) proteins will then be expressed in E. coli. I will therefore be grateful if anybody could recommend a suitable DNA codon for...

03 March 2013 7,584 1 View

I want to fuse MIC-A and ScFv with a linker, but I am confused about the construction. Please can you help? Should it be MICA-ScFv or ScFv-MICA?

I want to express these fused proteins and need to know which of the proteins should come first. This is to guide me in the primer design. MIC-A is a ligand of NKG2D and ScFv is a single chain...

01 January 2013 9,709 2 View

Will His-tag affect the bioactivity of a protein if it is attached to the N-terminus of that protein.

I am studying the bioactivy of two fused proteins. In order for the fused proteins to retain their respective bioactivities, I have decided to fuse them with a linker at their C-termini. I still...

01 January 2013 6,452 18 View