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Questions related from Desmond Omane Acheampong
MICA and its receptor NKG2D were expressed as an inclusion bodies in E coli. It was therefore denatured and renatured employing dialysis method of refolding. Carrying out ELISA of MICA and it...
01 January 2014 7,647 7 View
I am trying to solubilize and refold an inclusion body of NKG2D with theoretical isoelectric point (PI) value of 6.2. I usually purify my solubilized protein with Nickel column (Elution buffer pH...
12 December 2013 9,856 5 View
I want to insert my recombinant DNA of about 1650bp into pET22b which is about 5500bp. The resultant product from the ligation will be used for E. coli transformation. I seem not to get a...
09 September 2013 10,026 12 View
NKG2D is a receptor expressed on NK and T cells. I would be grateful if anybody could suggest a suitable cell line that can express NKG2D on its surface for in vitro studies.
03 March 2013 8,052 0 View
I want to link two proteins with the linker (G4S)3. The linked (fused) proteins will then be expressed in E. coli. I will therefore be grateful if anybody could recommend a suitable DNA codon for...
03 March 2013 7,582 1 View
I want to express these fused proteins and need to know which of the proteins should come first. This is to guide me in the primer design. MIC-A is a ligand of NKG2D and ScFv is a single chain...
01 January 2013 9,707 2 View
I am studying the bioactivy of two fused proteins. In order for the fused proteins to retain their respective bioactivities, I have decided to fuse them with a linker at their C-termini. I still...
01 January 2013 6,450 18 View