What is the best way to test the binding affinity of a Ligand (MICA) and its receptor (NKG2D) expressed in E coli?

MICA and its receptor NKG2D were expressed as an inclusion bodies in E coli. It was therefore denatured and renatured employing dialysis method of refolding. Carrying out ELISA of MICA and it...

31 December 2013 7,562 7 View

What pH of refolding buffer will be suitable for refolding (renaturing) a solubilized Inclusion Body of protein with isoelectric point (PI) 6.2?

I am trying to solubilize and refold an inclusion body of NKG2D with theoretical isoelectric point (PI) value of 6.2. I usually purify my solubilized protein with Nickel column (Elution buffer pH...

11 December 2013 9,778 5 View

Can any one help me with a suitable protocol for double digestion of a vector(pET22b) and an insert (DNA) with BAHM1 and NOT1 restriction enzymes?

I want to insert my recombinant DNA of about 1650bp into pET22b which is about 5500bp. The resultant product from the ligation will be used for E. coli transformation. I seem not to get a...

08 September 2013 9,957 12 View

Suitable DNA codon for the linker (G4S)3 for expression in E. coli?

I want to link two proteins with the linker (G4S)3. The linked (fused) proteins will then be expressed in E. coli. I will therefore be grateful if anybody could recommend a suitable DNA codon for...

02 March 2013 7,507 1 View

I want to fuse MIC-A and ScFv with a linker, but I am confused about the construction. Please can you help? Should it be MICA-ScFv or ScFv-MICA?

I want to express these fused proteins and need to know which of the proteins should come first. This is to guide me in the primer design. MIC-A is a ligand of NKG2D and ScFv is a single chain...

31 December 2012 9,644 2 View

Will His-tag affect the bioactivity of a protein if it is attached to the N-terminus of that protein.

I am studying the bioactivy of two fused proteins. In order for the fused proteins to retain their respective bioactivities, I have decided to fuse them with a linker at their C-termini. I still...

31 December 2012 6,366 18 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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03 March 2021 5,360 2 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

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02 March 2021 3,060 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View