Dear Imad, you can store SDS-PAGE resolving gel at 4 C without removing water layer on it. We cover gel with stretch film to prevent evaporation of water. Next day, you can pour off water and prepare stacking gel.

Best regards.

Dear Imad, you can store SDS-PAGE resolving gel at 4 C without removing water layer on it. We cover gel with stretch film to prevent evaporation of water. Next day, you can pour off water and prepare stacking gel.

Best regards.

Yes, it will be OK for most purposes.

Hope it helps!

There is a wealth of guidance in the attached papers. Best regards

Hello Imad: Yes, you can use it today if the water didn't evaporate. The next time you can try using a plastic container like a small tupperware with a lid an put it in the refrigerator just to be sure.I hope it helped :)

Dear imad, i thank you can do it but Preferred keeped in 4C.

Dear Imax

Your gel should be fine. Just remember the next time you prepare a gel to store at 4°C.

Hi Imad,

I do store my SDS-PAGE gels overnight often, however I use two different methods for storing. I make the resolving and stacking gels with the comb completely. If I will be using the gel the next day, I will leave the gel in the casting apparatus with the comb, add a layer of Tris buffer (1 M, pH 6.8) used for the stacking gel over top to maintain the pH, and then refrigerate at 4 degrees C. If I will be using the gel after more than one day, I will remove the comb and wrap it with a wet paper towel and then plastic wrap it and place the gel at 4 degrees C.

Thus far, I have not had any problems with the resolution of the gel or how the gel runs after storage. Hope this also works for you!

Best of luck,

Meera

hi Ed

You can store your SDS PAGE gel at 4C by covering with wet tissue paper and then covering with aluminium foil.