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Questions related from Ed Abraham
I poured the resolving gel for a western blot without pouring the stacking gel. I left the gel with DI water on top overnight at room temperature. Is it good to use today?
27 March 2018 7,610 9 View
The gel runs nicely, all the bands are nice and clear. I guess something happens during the transfer... the ladder looks fine but the lanes look fuzzy. After developing the blot, it's hard to see...
01 January 1970 8,259 5 View
In theory, TORC1 deletion should cause sphingolipid decrease because TORC1 inactivates orm1/2 (whicy are sphingolipid inhibitors). However, one often-cited paper is that TORC1 inhibition increases...
01 January 1970 8,951 0 View
