Could you recommend a PhosFlow-compatible human CD27 antibody?

I am measuring phosphorylated STAT5 in different lymphocyte populations using flow cytometry in response to stimulus. I need to accurately gate different memory populations using CD27 (not CCR7,...

30 July 2020 3,378 2 View

FlowAI and FlowClean plugins for data QC in FlowJo- could you help troubleshoot please?

I am analysing a 14-parameter flow cytometry panel in FlowJo v10.3 and would like to clean up the data before analysis. There are two plugins (flowClean and FlowAI) which use R to get rid of bad...

06 February 2020 9,566 3 View

Low ESS value for gammashape (BEAST.v.2)?

Hi all, I have some question specifically about a Beast analysis I am trying to run (I am a beginner). My dataset contains 2 mtDNA genes (COi and 16S) and 2 nuclear DNA genes (H3, 18S). I...

16 December 2019 460 3 View

What is your opinion about the regulatory issues of any research projects results on nanotoxicology and the emerging technologies?

Emerging technologies such as nanotechnology aim to improve our quality of life and to contribute to industrial competitiveness. The rapid scientific advance and the wide number of new...

24 November 2019 5,164 3 View

What PCR kit to use with degenerate primers?

I have to perform a PCR reaction with degenerate inosine containing primers, for virus typing purposes. I have the primers and protocol aviable for degenerate conditions. But in the manuel of PCR...

23 August 2019 9,088 3 View

Selective pressure analysis interpetation of the results?

I've been told to make a selective pressure analysis of a hypervariable region of a viral genome. I have aligned my sequences, removed stop codons and I did some tests I've met in publications...

28 February 2018 8,432 6 View

Stimulation of cancer cells with INF-gamma?

Does anyone have a protocol for stimulating cancer cells with IFN-gamma to promote CXCL10 production?

27 August 2017 5,405 3 View

How can I interpret the Likelihood ratio for a Chi-square test (SPSS)?

Hi all, I am performing a questionnaire analysis in SPSS using the Chi-square test (ordinal-ordinal, nominal-nominal, and ordinal-nominal comparison). However, my data does not meet the assumption...

15 April 2017 6,625 12 View

What is the best way to analyse a questionnaire with different types of data?

21 March 2017 7,981 4 View

Does anyone have experience with DNMT1 western-blot on nitrocellulose membrane?

Dear all, I am trying to do western-blot for DNMT1 in cells treated with Decitabine and untreated cells and I am using a nitrocellulose membrane but I am getting a lot of non-specific staining....

09 November 2016 2,489 4 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How much cell pressure and back pressure should I apply to start for triaxial test for different types of soil?

We have recently purchased a triaxial test instrument (MATEST) for testing different types of soil. After installing software, we are facing a issue of saturation where the samples does not...

23 February 2021 7,194 4 View

What will be the possible analysis plan for descriptive outcome?

Hi everyone I am busy with a Systematic review. Unfortunately, my protocol has been returned back because of my analysis plan that will not answer my question. Can you please advise on the...

02 February 2021 6,190 2 View

Are there any solutions for remote eye-tracking?

Since with the pandemic it doesn't look like we are going back to the lab soon, in my lab we were looking for solutions to conduct remote webcam-based eye-tracking. I've been looking at...

31 January 2021 7,315 5 View

How to extract protein after trizol RNA and back extraction of genomic DNA?

I have limited amount of samples, so I am trying to get RNA, DNA and protein from the same samples. I used Trizol reagent for RNA isolation, then use back extraction buffer for genomic DNA...

13 January 2021 1,566 2 View

Are most-recent papers of randomized clinical trials needed to conduct a legitimate meta-analysis?

Are most-recent papers of randomized clinical trials needed to conduct a legitimate meta-analysis or decades back will still provide optimum results?

28 December 2020 9,258 3 View

I want to use multilayer feedforward neural network with error backpropagation algorithm. I have just 30 years monthly rainfall data. Is this enough??

I am new to neural networks and want to apply multilayer feedforward neural network with error back propagation algorithm for the rainfall prediction. I have just monthly rainfall data of 30...

28 December 2020 9,755 4 View

Can language alter our perception of crime?

I am looking for any resources which may be useful in a study I am conducting on the impacts that language may have on our perception of crimes. I will be using headlines which convey a particular...

23 December 2020 8,713 6 View

Is ag paste suitable for back contact on silicon wafer? or i should deposit al on the back surface using pvd or plasma sputtering?

Fabricating silicon solar cell

22 December 2020 4,906 7 View

Fluorescence spectra problem during protein ligand interaction??

I checked protein ligand interaction through Fluroscence, I keep my protein concentration constant with varying concentration of ligand at the small concentration 5uM the FLuroscence is peak is...

21 December 2020 8,609 4 View