Pavlina Spiliopoulou

3 Questions 4 Answers 0 Followers

Questions related from Pavlina Spiliopoulou

Stimulation of cancer cells with INF-gamma?

Does anyone have a protocol for stimulating cancer cells with IFN-gamma to promote CXCL10 production?

28 August 2017 5,467 3 View

Does anyone have experience with DNMT1 western-blot on nitrocellulose membrane?

Dear all, I am trying to do western-blot for DNMT1 in cells treated with Decitabine and untreated cells and I am using a nitrocellulose membrane but I am getting a lot of non-specific staining....

10 November 2016 2,564 4 View

Does anyone know what is the Edu incubation dose for ID8 cells when trying to do Edu/propidium iodide assay for cell cycle/viability assessment?

Hi, I want to do a cycle/viability assay for ID8 ovarian cancer cells after treatment with the epigenetic drug Decitabine, on various doses. Has anyone done it and if yes, do you know the optimal...

20 October 2016 4,329 3 View