Hi, I want to do a cycle/viability assay for ID8 ovarian cancer cells after treatment with the epigenetic drug Decitabine, on various doses. Has anyone done it and if yes, do you know the optimal dose for Edu? And does Edu incubation need to take place before PI incubation? Thank you, Pavlina.
Hi Pavlina,I tested 10uM
I tested 10uM concentration for different cell lines for 1 h and a half. It has given me very good results in a combination with PI staining (20ug/ml).
If it does not work well, you can either double the Edu concentration or incubation time.
Hope it works for you properly.
Dear Hossein,
thank you very much for your advice. I will end up using BrdU in the end but it is nice to have your message for future reference. Many thanks again,
Pavlina
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