Postnatal stem cells have been isolated from a variety of tissues including bone marrow, brain, skin, hair follicles, skeletal muscle and dental pulp. These cells are thought to possess great therapeutic potential for repairing damaged and/or defective tissues. It has been shown that stem cells from human exfoliated deciduous teeth (SHED) represent a population of postnatal stem cells capable of extensive proliferation and multipotential differentiation. The transition from deciduous to permanent teeth is a very unique and dynamic process in which the development and eruption of permanent teeth synchronize with the resorption of the roots of deciduous teeth. The exfoliated deciduous tooth is similar in some ways to an umbilical cord, containing stem cells that may offer a unique postnatal stem cell source for potential clinical applications
Stem cells from apical papilla, stem cells from human exgoliated deciduous teeth, periodontal ligament stem cells and dental pulp stem cells have the potential to regenerate dental pulp along with the blood. They are said to have FGF, TGF, IGF. They population doubling of various forms of stem cells are different, though they are said to have a positive influence in regenerative endodontic therapies and tissue engineering. And studies have shown they are capable of forming cells similar to DPC.
I respect and appreciate this discussion, but may add
Differentiation ability is another key character of stem cell, so does DPSCs. As one kind of adult stem cells, the most key differentiation potential is the purpose of tissue or cells itself. To DPSCs, it means odontoblasts. After transplanting into immune-compromised mice, human DPSCs will automatically differentiate into the dentin-like structure with odontoblast-like cells. By seeding on chemically and mechanically treated dentin surface, DPSC-derived tissues also showed odontoblast-like morphology formation with dentinal tubule through scanning electron microscopy. Additionally, the increase of dentin matrix component-related genes, such as dentin matrix protein 1 (DMP-1) and dentin sialophosphoprotein (DSPP) expression double, confirmed odontoblasts differentiation results . In the process of odontoblasts differentiation, research showed the notch signaling pathway may help to induced angiogenesis and differentiate to regenerate vascularized dental pulp
What is the antibiotics which ican used instead of Amphotercin to prevent the contamination of stem cell media
contamination is everywhere. For Pluripotent stem cell culture, there are two things, in my opinion, that lead to even more risks of contamination:
- The fact that differentiation is often altered by antibiotics
- The fact that most of the time, you need to coat your culture dishes with an extracellular matrix protein, which can be easily contaminated by bacteria.
I would say that the worse are gelatin and Matrigel or any equivalent, because they gel. Therefore, any contamination is trapped together with the matrix, reducing the action of antibiotics during a amplification phase.
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