Can anybody share a stem cell culture protocol with me?
How to initiate and maintain the culture properly
Kindly guide me if any suitable protocols are there to study the interaction
29 April 2024 4,228 3 View
Being a researcher in the field of swarm intelligence for more than seven years, I still haven't found any research group/ institution or research lab dedicated to swarm intelligence research....
26 April 2024 4,415 2 View
TCR is tumor cell receptor positive macrophages which induces inflammation in cancer cell.
07 December 2023 7,299 2 View
For analysis of sugar beet juice quality, what formula is used to calculate commercial beet sugars and invert sugars
27 November 2023 5,721 0 View
CTC( Circulating tumour cells) are defined as tumour cells and at the time of metastasis, these specialised cells come into the circulation which can help to detect the cancer. Kindly provide me...
23 November 2023 2,471 2 View
How much impact of a startup failure comes on Its employee's financial and mental health.
29 August 2023 3,592 0 View
Is there any list or work or survey on current difficult or hard Computer Vison problems which are yet to solve efficiently?
12 August 2023 5,276 2 View
i have publications listed that are not mine but someone with the same name
17 April 2023 5,515 1 View
I have estimated proline in sugar beet leaves and have observed the absorbance at 520 nm. Now I want to make a standard curve for further estimation and calculation of proline. Can anyone help me...
01 June 2022 8,482 7 View
I'm designing a PDMS fluidics device that has millimeter-wide channels (~1-2mm wide), and I'd like to put inlets on it. The PDMS layer is 5-6 mm thick and is plasma bonded to glass. So far, I've...
24 July 2021 8,805 3 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View