Is there any matlab code available for 28 test functions at CEC'13 Special Session on Real-Parameter Optimization?

I am currently working on PSO where i need to test my algorithm with benchmark optimization test functions. Liang et al publish this paper ( Problem Definitions and Evaluation Criteria for the CEC...

29 July 2020 5,123 2 View

Why does the transmission spectra shows an increase near the optical absorption edge?

Measuring a transmission spectrum of thin film (eg: single phase oxide) using UV-Vis spectroscopy, the transmission value gradually increases as we decrease the wavelength and reaches a peak until...

30 April 2020 700 1 View

Do you have GC-MS datasets that are not analyzed/ published yet?

Hello, I am looking for unanalyzed and unpublished GC-MS datasets from diverse mass spectrometer vendors ? Such as ToFs, single/ triple Quads, Orbitraps etc. that have been acquired from human,...

10 April 2020 2,347 10 View

Which fluorescent markers I can use to stain live dead cells treated with doxorubicin?

Hi, Which is the best live, dead flourescent stain to use if I have to image a spheroid treated with Doxorubicin. The standard live /dead assay kit has Calcien-AM (live) and ethidium bromide...

22 May 2019 7,668 1 View

What is the best tool to identify and provide a good pictorial representation of homologous DNA sequences between species?

I want to identify homologous DNA sequences between mouse chicken, xenopus and zebrafish for a human non coding genomic regions. Any suggestion of which tool to use. I would prefer the one that...

03 April 2018 2,394 3 View

Why do metal-oxide composites show higher permittivity/refractive index as compared to pure oxide?

Since metals (eg: Au) show negative permittivity, the metal oxide composite should should show lower permittivity compared to the pure oxide phase. But standard methods such as Effective Medium...

19 February 2018 6,009 1 View

What could be the probable reason of getting a ring during biofilm formation in unicoluted media?

I have been trying to optimize the biofilm formation for enterobacter cloacae for which the biofilm formation is sparsely studied. i have been facing great difficulty in the C.V assy. I am...

11 February 2018 3,910 6 View

Which seems to be a better option: using Plasma or Serum, for determining blood based protein biomarkers using MALDI-TOF & 2D?

In a study, we need to determine blood based protein biomarkers in stroke using proteomics techniques like MALDI-TOF-MS and 2D gel electrophoresis. So, which component of the blood should we use...

15 November 2016 304 3 View

Which ELISA method would be most suitable to assay the human serum samples of C-Reactive Protein (CRP) ?

25 April 2015 2,332 2 View

One enzyme with higher superior catalytic activity or many enzymes, which one is favored ?

During the course of evolution, for functional (enzymatic proteins) which has been selected more favorably ? An enzyme with low to high catalytic activity (kinetics, rate,...

30 January 2015 7,603 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View