Is it feasible to co-culture TCR positive macrophages with myeloid cells? If yes, could someone provide a protocol detailing how to conduct this?

More Sanchaita Misra's questions See All

I aim to create a co-culture model to see the interaction between T lymphocytes and malignant cells, specifically targeting OSCC,is it possible?

Kindly guide me if any suitable protocols are there to study the interaction

29 April 2024 4,228 3 View

Is there any research group or lab that works on Swarm Intelligence?

Being a researcher in the field of swarm intelligence for more than seven years, I still haven't found any research group/ institution or research lab dedicated to swarm intelligence research....

26 April 2024 4,415 2 View

What is the formula for commercial beet sugars and invert sugars in sugar beet?

For analysis of sugar beet juice quality, what formula is used to calculate commercial beet sugars and invert sugars

27 November 2023 5,721 0 View

How can we effectively isolate Circulating Tumor cell from whole blood?

CTC( Circulating tumour cells) are defined as tumour cells and at the time of metastasis, these specialised cells come into the circulation which can help to detect the cancer. Kindly provide me...

23 November 2023 2,471 2 View

Impact of Startup success or Failure ?

How much impact of a startup failure comes on Its employee's financial and mental health.

29 August 2023 3,592 0 View

What are the computer vison problems yet to efficiently solve up to 2023?

Is there any list or work or survey on current difficult or hard Computer Vison problems which are yet to solve efficiently?

12 August 2023 5,276 2 View

How do i remove publications that are not mine on research gate but someone with the same name?

i have publications listed that are not mine but someone with the same name

17 April 2023 5,515 1 View

How to make a standard curve for proline estimation in sugar beet?

I have estimated proline in sugar beet leaves and have observed the absorbance at 520 nm. Now I want to make a standard curve for further estimation and calculation of proline. Can anyone help me...

01 June 2022 8,482 7 View

How to add inlets to PDMS fluidics device?

I'm designing a PDMS fluidics device that has millimeter-wide channels (~1-2mm wide), and I'd like to put inlets on it. The PDMS layer is 5-6 mm thick and is plasma bonded to glass. So far, I've...

24 July 2021 8,805 3 View

Can anyone help me on a list of journals based on application of swarm intelligence?

I need a list of journals SCI indexed based on application of swarm intelligence.

18 July 2021 6,028 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

How long will macrophages remain in the M1 state after treatment with 100 ng/mL of LPS?

In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...

01 August 2024 476 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View