What's the difference between 0.1% triton-X100 and ice-cold methanol in the immunofluorescence assay for intracellular protein staining?

Hi, Guys, I noticed that researchers usually permeabilized the cells with 0.1% Triton-X100 or ice-cold methanol (90%) for an intracellular protein staining, it looks like that the two methods work...

15 February 2021 5,606 4 View

How to Draw Crystal Structure from powder XRD?

Hi, I would appreciate if anyone can recommend on the process of drawing crystal structure from observed powder xrd diffractogram and if there is any suggested software to work on.

05 January 2021 3,517 3 View

Does PFA transcardial perfusion artefactually stretch the rodent heart?

Hi everyone I'm comparing aorta/ventricle wall thickness of mouse. Perfusion was done to minimise auto fluorescence during confocal IF. Question is whether transcardial perfusion artefactually...

28 October 2020 8,059 2 View

How to wash the LiF left by Ti3AlC2 etching?

I added 1 g Ti3AlC2 to 1g LiF and 20mL 9 M HCl and stirred it at 450 rpm at room temperature for 48 h.After deionized water washing and ultrasonic treatment, the product was obtained, but its XRD...

08 October 2020 7,612 4 View

Is this typical lymphocyte, the flower cells?

21 September 2020 4,107 3 View

How is the quality of archived blood sample for HbA1c test?

HbA1c is an important indicator of long-term glycemic control with the ability to reflect the cumulative glycemic history of the preceding two to three months. Also, HbA1c is measured as the ratio...

16 September 2020 2,423 4 View

Which is better in determining the effectiveness of artificial licks for wildlife detection in forested areas?

Hello, I'm planning for my future research study on artificial salt lick. The study site that I've selected for this study has been confirmed with no presence of natural salt lick, thus I plan to...

02 August 2020 5,609 1 View

What statistical tests can I perform on glycomics data collected from disease cohort across 3 timepoints?

I have a dataset comprising clinical characteristics and glycomics profiles of disease samples (n=100+) and matched healthy controls. So far, I have been able to do classification modeling to...

20 July 2020 2,153 3 View

0.5M EDTA buffer preparation: Why does it turn into milky white foam-like mixture?

I am making 200ml of 0.5M EDTA, pH8. I mixed 29.22g EDTA (MW: 292.24g/mol) with 100ml distilled water first (planning to add up to 200ml after measuring pH). I was adding NaOH pellet into the...

03 June 2020 4,581 3 View

Does anyone know the reason why an error occurs when creating a module using udms (user defined models) in psat?

I am using User Defined Models (UDMs) in PSAT to create a new type component . In order to deepen the understanding, I used examples come with PSAT (pmu)for modeling. No error when I click on...

31 May 2020 1,940 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View