Si Ying Lim

5 Questions 1 Answers 0 Followers

Questions related from Si Ying Lim

Stains (flow cytometry) needed for isolated red blood cells?

I have red blood cells isolated according to the protocol attached. I would like to know what stain would be required for flow cytometry to identify the red blood cells for gating. Also, if I want...

17 September 2021 1,903 1 View

Can you tell me what the spiky cells are in my sample of red blood cell extract (confocal microscopy)?

Context: Isolated red blood cells (protocol in .docx is attached) Did confocal microscopy (stained RBCs with CellMask Red (photoiodide)) Observed red blood cells (circled in blue) and also some...

06 September 2021 9,493 2 View

Recommended method / product for platelets cell counting?

I am in a lab without microscopes. Can anyone recommend an affordable method / product for cell counting of human platelets (~1.5 - 3um)? Thank you!

06 July 2021 7,345 4 View

Isolating non-activated and non-aggregated platelets for functional or "single-cell" analysis?

Question 1: I would like to isolate platelets without activating them beforehand and also at the same time make sure that I can analyse them in non-aggregated forms (i.e. Individual platelets)....

22 January 2021 6,097 1 View

What statistical tests can I perform on glycomics data collected from disease cohort across 3 timepoints?

I have a dataset comprising clinical characteristics and glycomics profiles of disease samples (n=100+) and matched healthy controls. So far, I have been able to do classification modeling to...

21 July 2020 2,407 3 View