Are you a researcher or practicioner in the climate change adaptation field? Do you have knowledge or experience with Monitoring and Evaluation?

Dear Colleagues, My name is Sylvia Lima and I am conducting a survey as part of my Ph.D. research in evaluation of climate change adaptation success in coastal areas of developing countries with...

09 November 2020 6,434 3 View

LED light for plant growth chamber for ecotoxicity experiements - which set up and typ of lighting do you recommend?

We are building a new plant growth chamber for plant ecotoxicity experiments (algae, seed germination, genotox experiment with Vicia faba, Lemna growth inhibition etc. The old one used cooling and...

21 June 2020 8,017 2 View

Statistical test for conditional regression?

Is there any statistical test or work-around to test whether the effect of variable a on c increases if b is high? All variables were asked for on a 7-point likert scale. I thought about...

17 July 2019 3,973 2 View

PMMA dissolved in acetone, VOCs released?

Acetone is a solvent for PMMA. Could anyone direct me to articles where the VOCs that are released during the formation of the homogeneous solution?

05 June 2018 9,051 2 View

Effect size from LOESS plots?

Is it possible to obtain an effect size (such as correlation coefficient or some measure of goodness of fit) from a locally weighted scatterplot smoothing plot? The reason for obtaining an effect...

23 October 2017 9,865 1 View

In a regression analysis with a large number of ordinal predictors, how can I test linearity? What plots may be specific to those ordinal variables?

I have close to 50 predictors in my regression analysis and I have no problem testing the linearity of the interval covariates but I am unsure if the plots I have produced for the interval ones...

18 March 2017 1,356 12 View

How can I get my porcine chondrocytes to detatch from 24 well plates after 7 days of culture?

18 August 2016 7,366 3 View

Can anybody recommend a good antibody against Jun for WB and ChIP in rat?

Can anybody recommend a good antibody against Jun for WB and ChIP in rat? Thanks.

06 June 2016 4,247 2 View

Does SDS add significant ionic strength to transfer buffer when doing a wet Western transfer?

I am doing a wet Western transfer and yesterday used Transfer buffer (1X Tris-Glycine) and today did the same exact Western transfer protocol but with 0.1% SDS Transfer buffer. Yesterday, I ran it...

18 May 2016 1,763 4 View

Is there an alternative to Argon gassing for adequate storage of lipids?

Hello Colleagues, I have vials of a phospholipid formulation that I will use to prepare lipososomes. Once opened, I will not be using each vial immediately. Most researchers report using something...

07 April 2016 2,376 2 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

Specify the Burgers vectors for dislocations, which cannot be uniquely identified.?

1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...

20 February 2021 2,370 1 View

I am working on image transmission, i converted the image into binary vector, i want to extract the MSB of each pixel ?

i have a vector 1*512 size, i want to take the first value every 8 elements and put these elements at the end of the vector,how to do this on matlab ?

19 February 2021 2,352 5 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

Can I express protein of plant origin in E. coli strain (M15) using pET28a vector systems? Are M15 strains and pET vector systems compatible?

Are E.coli strains M15 and pET28a vector systems compatible? Can I over-express my protein which is of plant origin. Previously I was using BL21 DE3 strains. Was confused to use M15 strains for...

10 February 2021 2,564 3 View

Plasmid amplification in S2 cell?

When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...

09 February 2021 5,423 1 View

Why cloning of gene into pCAMBIA1304 vector is unsuccessful?

I have tried to clone a specific gene into pCAMBIA1304 vector. But all the attempts failed. I used ligation kit from both Promega Corporation and Takara Bio. However, there was one time I used...

04 February 2021 525 1 View