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Dear all, I want to construct a DIO (double-floxed inverse orientation) vector with two transgenes flanked by two sets of different lox sites (pls see image). Hence, after addition of Cre...
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I read the table about cleavage close to the end of DNA fragments provided on the NEB website. According to that table efficiency of NotI cleavage of short DNA fragments is 0%. I'm asking 'cause I...
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