Hi all,
Does anyone know of a kit for measuring HMG coA synthase in cell lysates? I will prefer a non-radioactive kit that can be used with a spectrophotometer.
Thanks in advance.
Sylvia
There's not much on the market. I don't know of any kits for measuring enzyme activity.
The only ones I've found are either histology kits or ELISA kits.
- One first : HMGCS1 / HMG-CoA Synthase 1 Histology Assay Kit (CAT#: ITS-1122-ZP1795). Perhaps, it can be used for cells fixed on a plate, as is often the case with this type of kit. But I don't know its sensitivity on cells.
Reactivity: Human; Mouse; Rat
Sample Type :Tissue.
- Another one : Human HMGCS1 / HMG-CoA Synthase 1 (Sandwich ELISA) ELISA Kit - LS-F33086
Reactivity: Human
Range: 0.156-10 ng/ml
Colorimetric - 450nm (TMB)
This kit is a priori dedicated to plasma and serum, but often they have not been validated for cell lysates, test can be made.
Good luck with your search.
All the best
Marc
Hello Dr Conti,
Thank you very much for your response. If I use ELISA, I guess I will be limited to only knowing the amount of HMGCS1 in my cell lysates. I am actually interested in measuring the activity of HMGCS1 in my cell lysates. I will be grateful if you have suggestions for this as well.
Thanks in advance.
Dear Sylvia,
unfortunately, I haven't found any publications explaining how to measure this activity in cell lysates.
Sorry for the inconvenience.
I hope someone else can help you.
Marc