I have stored my samples in 50 % glycerol for two months, I am now ready to do the DNA extraction using Bioline Isolate II genomic DNA kit. How does the 50 % glycerol affect the DNA purity or quality in the samples? kindly assist.
Dear Colleagues, My name is Sylvia Lima and I am conducting a survey as part of my Ph.D. research in evaluation of climate change adaptation success in coastal areas of developing countries with...
09 November 2020 6,434 3 View
We are building a new plant growth chamber for plant ecotoxicity experiments (algae, seed germination, genotox experiment with Vicia faba, Lemna growth inhibition etc. The old one used cooling and...
21 June 2020 8,017 2 View
Acetone is a solvent for PMMA. Could anyone direct me to articles where the VOCs that are released during the formation of the homogeneous solution?
05 June 2018 9,051 2 View
Is it possible to obtain an effect size (such as correlation coefficient or some measure of goodness of fit) from a locally weighted scatterplot smoothing plot? The reason for obtaining an effect...
23 October 2017 9,865 1 View
I have close to 50 predictors in my regression analysis and I have no problem testing the linearity of the interval covariates but I am unsure if the plots I have produced for the interval ones...
18 March 2017 1,356 12 View
18 August 2016 7,366 3 View
Can anybody recommend a good antibody against Jun for WB and ChIP in rat? Thanks.
06 June 2016 4,247 2 View
Hello Colleagues, I have vials of a phospholipid formulation that I will use to prepare lipososomes. Once opened, I will not be using each vial immediately. Most researchers report using something...
07 April 2016 2,376 2 View
Hello - Can anyone please direct me to a reference that may give morphometry data on mouse oocytes, both at GV and MII stages? Basically I have an idea that the mouse oocyte measures at 70 um in...
09 February 2015 9,659 4 View
09 December 2014 9,729 6 View
I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...
02 March 2021 2,735 2 View
I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.
01 March 2021 4,306 1 View
I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...
01 March 2021 9,525 4 View
I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...
01 March 2021 7,675 3 View
I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...
01 March 2021 8,079 2 View
The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.
28 February 2021 9,464 2 View
I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...
28 February 2021 7,330 4 View
28 February 2021 8,466 2 View
Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...
28 February 2021 9,138 2 View
Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...
28 February 2021 912 3 View