Are you a researcher or practicioner in the climate change adaptation field? Do you have knowledge or experience with Monitoring and Evaluation?

Dear Colleagues, My name is Sylvia Lima and I am conducting a survey as part of my Ph.D. research in evaluation of climate change adaptation success in coastal areas of developing countries with...

09 November 2020 6,434 3 View

LED light for plant growth chamber for ecotoxicity experiements - which set up and typ of lighting do you recommend?

We are building a new plant growth chamber for plant ecotoxicity experiments (algae, seed germination, genotox experiment with Vicia faba, Lemna growth inhibition etc. The old one used cooling and...

21 June 2020 8,017 2 View

PMMA dissolved in acetone, VOCs released?

Acetone is a solvent for PMMA. Could anyone direct me to articles where the VOCs that are released during the formation of the homogeneous solution?

05 June 2018 9,051 2 View

Effect size from LOESS plots?

Is it possible to obtain an effect size (such as correlation coefficient or some measure of goodness of fit) from a locally weighted scatterplot smoothing plot? The reason for obtaining an effect...

23 October 2017 9,865 1 View

In a regression analysis with a large number of ordinal predictors, how can I test linearity? What plots may be specific to those ordinal variables?

I have close to 50 predictors in my regression analysis and I have no problem testing the linearity of the interval covariates but I am unsure if the plots I have produced for the interval ones...

18 March 2017 1,356 12 View

How can I get my porcine chondrocytes to detatch from 24 well plates after 7 days of culture?

18 August 2016 7,366 3 View

Can anybody recommend a good antibody against Jun for WB and ChIP in rat?

Can anybody recommend a good antibody against Jun for WB and ChIP in rat? Thanks.

06 June 2016 4,247 2 View

Is there an alternative to Argon gassing for adequate storage of lipids?

Hello Colleagues, I have vials of a phospholipid formulation that I will use to prepare lipososomes. Once opened, I will not be using each vial immediately. Most researchers report using something...

07 April 2016 2,376 2 View

What is the exact size and range for mouse oocytes?

Hello - Can anyone please direct me to a reference that may give morphometry data on mouse oocytes, both at GV and MII stages? Basically I have an idea that the mouse oocyte measures at 70 um in...

09 February 2015 9,659 4 View

What would cause good R2 value but bad Ct and efficiency in real time pcr?

09 December 2014 9,729 6 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How to find the mode of classified ocean colour images?

Hello, I have classified 10 S3 OLCI images from the same area (althouth not the same size and/or quadrant) and I would like to do a final image using the mode of the pixel of these 10 images. I...

01 March 2021 9,874 3 View

How to threshold an image despite bright lighting, using ImageJ/Fiji?

Hi everyone, I am using Fiji to determine the percentage of the image covered by leaves, branches, or trunk, but i've encountered some difficulty when the sun hits parts of my image like the one...

27 February 2021 1,194 3 View

Trouble with Charting with Google Earth Engine (Technical)?

Hello. I have a problem with the charting in google earth engine. I created an image collection by combining 5 image collections of five parameters and using ee.Join.inner ( ) and .apply ( )...

26 February 2021 7,084 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View