Sorting and counting of dendritic cells.

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Epigenetic modification and host controlled restriction modification:

Is there any evolutionary link between the host controlled restriction modification in bacteria (caused by methylation of bacterial sequences to protect viral entry) and methylation of nucleic...

02 March 2013 8,940 1 View

Is it possible to induce host controlled restriction modification in humans to prevent viral entry?

Host control restriction modification is mainly seen in micro organisms which helps them to protect themselves from invasion of virus. is it possible to induce same modificaton in higher organisms...

01 February 2013 4,966 0 View

Where can I get the list of unicellular organisms so that are so far identified? Can anyone help me with this?

Where can I get the list of unicellular organisms so that are so far identified?

10 November 2012 3,570 0 View

Circulating RNAs:

Hoe these circulating RNAs protect themselves from degradation in the circulating environment? Can any one help me with this? thank you so much.

07 August 2012 363 0 View

Is the amount of mRNA in a cell directly proportional to the level of protein coded by that mRNA?

Is the amount of mRNA in a cell directly proportional to the level of protein coded by that mRNA? Does an increase in mRNA level indicate an increase in the level of protein expression?

06 July 2012 7,670 15 View

RNA world

It is said by many researchers that the first compound formed was an amino acid. What theories explain the evolution to RNA?

06 July 2012 7,682 1 View

Transcription and Translation termination of Structural genes

It is said that the structural genes are transcribed continuously. For example lets take the lac operon; if the gene for galactosidase is fully transcribed then transcription should end to...

06 July 2012 2,850 5 View

Can eukaryotic mRNAs be transferred and translated in plants when injected as native DNA, without splicing?

I have a doubt about what happens when a mammalian DNA (not cDNA) is transferred to a plant and the extent of its expression. Will it get expressed? If it expresses then will that protein be a...

05 June 2012 3,868 18 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View