Where can I get the list of unicellular organisms so that are so far identified?
Is there any evolutionary link between the host controlled restriction modification in bacteria (caused by methylation of bacterial sequences to protect viral entry) and methylation of nucleic...
02 March 2013 8,940 1 View
Host control restriction modification is mainly seen in micro organisms which helps them to protect themselves from invasion of virus. is it possible to induce same modificaton in higher organisms...
01 February 2013 4,966 0 View
Can anyone suggest fluorescent dyes suitable for sorting of dendritic cells from PBMC cells using FACS?
01 February 2013 9,615 2 View
Hoe these circulating RNAs protect themselves from degradation in the circulating environment? Can any one help me with this? thank you so much.
07 August 2012 363 0 View
Is the amount of mRNA in a cell directly proportional to the level of protein coded by that mRNA? Does an increase in mRNA level indicate an increase in the level of protein expression?
06 July 2012 7,670 15 View
It is said by many researchers that the first compound formed was an amino acid. What theories explain the evolution to RNA?
06 July 2012 7,682 1 View
It is said that the structural genes are transcribed continuously. For example lets take the lac operon; if the gene for galactosidase is fully transcribed then transcription should end to...
06 July 2012 2,850 5 View
I have a doubt about what happens when a mammalian DNA (not cDNA) is transferred to a plant and the extent of its expression. Will it get expressed? If it expresses then will that protein be a...
05 June 2012 3,868 18 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?
02 August 2024 9,518 2 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View
Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.
28 July 2024 6,501 0 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...
22 July 2024 6,592 3 View