Hoe these circulating RNAs protect themselves from degradation in the circulating environment? Can any one help me with this? thank you so much.
Is there any evolutionary link between the host controlled restriction modification in bacteria (caused by methylation of bacterial sequences to protect viral entry) and methylation of nucleic...
02 March 2013 8,851 1 View
Host control restriction modification is mainly seen in micro organisms which helps them to protect themselves from invasion of virus. is it possible to induce same modificaton in higher organisms...
01 February 2013 4,878 0 View
Can anyone suggest fluorescent dyes suitable for sorting of dendritic cells from PBMC cells using FACS?
01 February 2013 9,518 2 View
Where can I get the list of unicellular organisms so that are so far identified?
10 November 2012 3,490 0 View
Is the amount of mRNA in a cell directly proportional to the level of protein coded by that mRNA? Does an increase in mRNA level indicate an increase in the level of protein expression?
06 July 2012 7,567 15 View
It is said by many researchers that the first compound formed was an amino acid. What theories explain the evolution to RNA?
06 July 2012 7,608 1 View
It is said that the structural genes are transcribed continuously. For example lets take the lac operon; if the gene for galactosidase is fully transcribed then transcription should end to...
06 July 2012 2,756 5 View
I have a doubt about what happens when a mammalian DNA (not cDNA) is transferred to a plant and the extent of its expression. Will it get expressed? If it expresses then will that protein be a...
05 June 2012 3,780 18 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?
24 February 2021 7,064 3 View
Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...
21 February 2021 6,633 3 View
I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...
17 February 2021 8,986 2 View
I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...
03 February 2021 9,330 3 View
I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...
02 February 2021 8,806 2 View
I'm working on selecting mouse miRNAs to analyze and stumbling upon one confusing issue, some of the papers do not specify which arm they analyzed and they just state something like " miR-34a was...
16 January 2021 3,792 4 View
I am trying to confirm my miRNA target by western blotting. I have a ds mimic and a ss inhibitor of my miRNA. Is it necessary to use negative controls for mimic and inhibitor? If yes, how can I...
09 January 2021 2,320 3 View
Hello! I am trying to predict in silico the miRNA by knowing the target. Most of published research starts by knowing the miRNA and predicts the target using online tools. Are you familiar with...
31 December 2020 7,433 5 View
I am searching for miRNA repeats put between WPRE and 3'LTR of my lentivirus, in order to enhance the specificity of transgene expression in human neuron cells. But if clinically translation of...
26 December 2020 9,921 2 View