Hoe these circulating RNAs protect themselves from degradation in the circulating environment? Can any one help me with this? thank you so much.
Is there any evolutionary link between the host controlled restriction modification in bacteria (caused by methylation of bacterial sequences to protect viral entry) and methylation of nucleic...
02 March 2013 8,940 1 View
Host control restriction modification is mainly seen in micro organisms which helps them to protect themselves from invasion of virus. is it possible to induce same modificaton in higher organisms...
01 February 2013 4,966 0 View
Can anyone suggest fluorescent dyes suitable for sorting of dendritic cells from PBMC cells using FACS?
01 February 2013 9,615 2 View
Where can I get the list of unicellular organisms so that are so far identified?
10 November 2012 3,570 0 View
Is the amount of mRNA in a cell directly proportional to the level of protein coded by that mRNA? Does an increase in mRNA level indicate an increase in the level of protein expression?
06 July 2012 7,670 15 View
It is said by many researchers that the first compound formed was an amino acid. What theories explain the evolution to RNA?
06 July 2012 7,682 1 View
It is said that the structural genes are transcribed continuously. For example lets take the lac operon; if the gene for galactosidase is fully transcribed then transcription should end to...
06 July 2012 2,850 5 View
I have a doubt about what happens when a mammalian DNA (not cDNA) is transferred to a plant and the extent of its expression. Will it get expressed? If it expresses then will that protein be a...
05 June 2012 3,868 18 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...
24 June 2024 3,507 2 View
Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...
18 June 2024 2,799 4 View
Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...
16 June 2024 9,723 2 View
Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...
06 June 2024 5,715 4 View
The size miRNA is 89 bps and the primers I have designed (using traditional method for designing gene primers) should give an amplified product of 71 bps. But I am getting product of higher...
23 May 2024 7,570 3 View
I have a question regarding target miRNA expression after transfection with antisense miRNA (miRNA inhibitor). I understand that the expression of the target miRNA will increase after mimic...
20 May 2024 5,920 4 View
Hello. Is there any webserver where I can input a set of transcription factors, genes, and miRNAs, and it can provide me with statistical data on how TFs either activate or repress genes and...
18 May 2024 5,848 1 View
Hello all. I have recently been trying to isolate total RNA after transfection of miRNA mimics and antisense inhibitors in mammalian cells. However, the RNA isolated through the trizol method...
13 May 2024 4,935 7 View
would like to work on microRNA to detect colorectal cancer. I will use the type of microRNA type micRNA124,micro143 how I can make primer design for it and how I can choose the primer ?
07 May 2024 8,812 2 View