Epigenetic modification and host controlled restriction modification:

Is there any evolutionary link between the host controlled restriction modification in bacteria (caused by methylation of bacterial sequences to protect viral entry) and methylation of nucleic...

02 March 2013 8,851 1 View

Sorting and counting of dendritic cells.

Can anyone suggest fluorescent dyes suitable for sorting of dendritic cells from PBMC cells using FACS?

01 February 2013 9,518 2 View

Where can I get the list of unicellular organisms so that are so far identified? Can anyone help me with this?

Where can I get the list of unicellular organisms so that are so far identified?

10 November 2012 3,490 0 View

Circulating RNAs:

Hoe these circulating RNAs protect themselves from degradation in the circulating environment? Can any one help me with this? thank you so much.

07 August 2012 265 0 View

Is the amount of mRNA in a cell directly proportional to the level of protein coded by that mRNA?

Is the amount of mRNA in a cell directly proportional to the level of protein coded by that mRNA? Does an increase in mRNA level indicate an increase in the level of protein expression?

06 July 2012 7,567 15 View

RNA world

It is said by many researchers that the first compound formed was an amino acid. What theories explain the evolution to RNA?

06 July 2012 7,608 1 View

Transcription and Translation termination of Structural genes

It is said that the structural genes are transcribed continuously. For example lets take the lac operon; if the gene for galactosidase is fully transcribed then transcription should end to...

06 July 2012 2,756 5 View

Can eukaryotic mRNAs be transferred and translated in plants when injected as native DNA, without splicing?

I have a doubt about what happens when a mammalian DNA (not cDNA) is transferred to a plant and the extent of its expression. Will it get expressed? If it expresses then will that protein be a...

05 June 2012 3,780 18 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View