Dario Armando Leone

2 Questions 11 Answers 0 Followers

Questions related from Dario Armando Leone

Is there an acid treatment that allows removal of plasma membrane protein ???

I would like to determine the relative percentage of a protein expressed at the cell surface as well as intracellularly and I remember that there is an acid wash treatment able to destroy membrane...

06 June 2016 298 1 View

Why do I lose 50%-60% of T-cells after staining with CFSE or CPD?

I am setting up an antigen proliferation assay using Monocytes Derived Dendritic pulsed with KLH in co-culture with lymphocytes isolated by elutriation technique. I perform a CD4 negative...

04 April 2013 5,474 20 View