Dear Dr. Jain,

I hope this is helpful,

Fluorography of tritium-labeled proteins in silver-stained polyacrylamide gels

https://www.sciencedirect.com/science/article/abs/pii/0003269788906689?via%3Dihub

Silver-staining of polyacrylamide gel electrophoresis (PAGE)-separated proteins allows sensitive detection of proteins but severely reduces the ability to detect weak β-emitters present in the protein band. A simple procedure is described in which silver can be removed from a silver-stained PAGE gel (deargentation) using photographic fixer, and the silver-free gel can be enhanced and used for fluorography. A quantitative study of sensitivity is reported for 3H-labeled bovine serum albumin with a one-dimensional sodium dodecyl sulfate-PAGE slab gel.

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